蝮蛇毒介导小鼠急性心肌损伤及心肌组织TNF-α表达的研究

目的探讨蝮蛇毒介导小鼠心肌组织中TNF-α蛋白表达变化与不同蝮蛇毒剂量、中毒时间的关系。方法确定LD50蝮蛇毒注射剂量。将96只小鼠随机分为空白对照组、蛇毒低剂量(0.5mg/kg)组、蛇毒高剂量(1.0mg/kg)组,并按中毒时间设4水平组,即3、6、24、48h组,采集小鼠心肌标本,免疫组化检测心肌组织中TNF-α蛋白的表达情况。结果蛇毒低剂量组及蛇毒高剂量组小鼠心肌TNF-α蛋白表达明显高于空白对照组(均P0.05);而蛇毒高剂量组明显高于低剂量组(P0.05);6h组明显高于3h组(P0.05);24h组高于6h组(P0.05);48h组高于24h组(P0.05)。随着蝮蛇毒剂量的增加、中毒时间的延长,心肌TNF-α蛋白的表达越高。结论蝮蛇毒导致小鼠急性心肌损伤,TNF-α蛋白表达增高,且随着蝮蛇毒剂量的增加、中毒时间的延长,心肌TNF-α蛋白的表达越高,呈一定的时间、剂量效应趋势,提示TNF-α蛋白过度表达可能是蝮蛇毒导致小鼠急性心肌损伤的机制之一。

Study of Acute Myocardial Injury and Expression of TNF-α on Myocardium Tissue of Mice Induced by Agkistrodon halys （Pallas） Venom

Objective Explore the relationship between expression of TNF-a on myocardium tissue of mice and different dose of venom,the poisoning time induced by Agkistrodon halys （Pallas） venom. Methods Using two- factor factorial design, the 96 male Kunming mice of clean grade were divided into 12 groups（n= 8） randomly. Snake venom was divided into three levels： 0-dose（0.0mg/kg）,low-dose （0.5 mg/kg）, high-dose （1.0 mg/kg） ; Poisoning time to set up four levels： 3 hours, 6 hours, 24 hours, 48 hours, detected TNF-α protein expression of my- ocardial tissue by Immunohistochemical. Results Monitored by immunohistochemistry in mice with myocardial expression of TNF-a protein was found that compared with the control group,experimental group of mice myocardial TNF-a protein expression was significantly increased, the difference was statistically significant （P〈0.05）. Conclusion With acute myocardial injury of mice induced by Agkistrodon halys （Pallas）venom, expression of TNF-e on myocardium tissue of mice was increased,and the increase of the dose of venom and the poisoning tithe, the more expression of TNF-α, showing a certain time, dose effect trend, suggesting that overexpression of TNF-α protein one of the mechanisms of acute myocardial injury induced by Agkistrodon halys （Pallas） venom.