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Transfer of genome fragments in double infection of E. coli with T5 and phi X174 bacteriophages. |
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| Authors: | J G Atherton |
| Affiliation: | Department of Microbiology, University of Queensland, St. Lucia, 4067 Brisbane, Queensland, Australia |
| Abstract: | Double infection of Escherichia coli by two DNA phages (phi X174 and T5) resulted in encapsidation into T5 particles of T5 DNA containing linked fragments of phi X174 DNA. The phi X474 sequences in T5 "hybrid" DNA were detected by RNA-DNA hybridization. |
| Keywords: | Encapsidation of joint DNA molecules naturally formed recombinant DNA molecular hybridization d/min disintegrations per minute (dpm) SDS sodium dodecyl sulfate SSC 0.15 M NaCl,0.015 M Na-citrate,pH, 7.2 cRNA complementary RNA (see MATERIALS AND METHODS) |
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