Cryopreservation of immature embryos of Theobroma cacao |
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Authors: | Valerie Creaser Pence |
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Institution: | (1) Center for Reproduction of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, 3400 Vine Street, 45220 Cincinnati, OH, USA |
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Abstract: | Immature, white zygotic embryos of Theobroma cacao L. (cacao) retained the ability to produce callus and to undergo somatic embryogenesis after slow hydrated freezing and desiccated fast freezing in liquid nitrogen. The highest rate of somatic embryogenesis occurred in embryos which were precultured on a medium containing 3% sucrose, frozen slowly with cryoprotectants before exposure to liquid nitrogen, and recovered on a medium containing 3 mg/liter NAA. Embryos precultured on media containing sucrose increasing to 21% had a higher rate of survival but were less embryogenic after freezing. These results suggest that immature embryos might be used for long-term germplasm storage of T. cacao germplasm. |
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Keywords: | Cryopreservation embryogenesis germplasm preservation recalcitrant seeds Theobroma cacao |
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