Herbicide-binding to thylakoid membranes of a DCMU-resistant mutant of Chlamydomonas reinhardii

The specific binding of DCMU and atrazine to the thylakoid membranes of a uniparentally inherited DCMU-resistant mutant dr-416 of Chlamydomonas reinhardii was measured. Whole cells of the mutant can tolerate a 15-fold concentration of DCMU as compared to the parent strain. The same tolerance is found for the photosystem II activity of isolated thylakoid membranes. The mutant is not resistant against atrazine. In equilibrium-binding studies with [¹⁴C]atrazine and unlabelled DCMU, the specific binding for atrazine was found to be identical in the mutant and in the parent strain. The competitive binding of DCMU is significantly weaker for membranes of the mutant than of the parent strain, the equilibrium dissociation constants being 2.0 × 10^?7 M and 3.8 × 10^?8 M, respectively.