Improved determination of urinary oxalate with alkylamine glass bound barley oxalate oxidase |
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Authors: | Chandran P Thakur M Pundir C S |
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Institution: | Biochemistry Research Laboratory, Department of Bio-Sciences, M.D. University, Rohtak-124001, Haryana, India. |
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Abstract: | The measurement of oxalate in urine was improved by employing barley oxalate oxidase immobilized on alkylamine glass beads affixed in a glass beaker. The minimum detection limit was 3.6 mg l(-1) urine. The recovery of added oxalate was 88.9+/-9.2%. Within- and between-assay coefficients of variation (CV) were <4.0 and <9.4%, respectively. The urinary oxalate values were obtained by a commercial kit method and the present method showed a good correlation (0.999). The method is free from tedious handling of glass beads and Cl- interference. |
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