Immunohistochemical localization of cyclic AMP and ultrastructural demonstration of adenylate cyclase activity in the testis of Esox lucius at time of spermiation |
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| Authors: | Prof Rita Pascolini P Ceccarelli A M Gargiulo S Lorvik |
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| Institution: | (1) Institute of Comparative Anatomy, Faculty of Science, University of Perugia, Perugia, Italy;(2) Institute of Normal Anatomy, Faculty of Veterinary Medicine, University of Perugia, Perugia, Italy;(3) Istituto di Anatomia Comparata, Via Pascoli, 06100 Perugia, Italy |
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| Abstract: | Summary In the testis of Esox lucius at the time of spermiation, activity of cyclic adenosine 3 ,5-monophosphate (cAMP) was immunocytochemically localized at the level of the Sertoli cells. In these cells adenylate cyclase activity was also ultracytochemically demonstrated by using adenylyl imidodiphosphate as a substrate. Reaction products of adenylate cyclase were primarily detectable on the basal and adluminal plasma membranes and on the surface of protrusions of the cell body into the lumen. |
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| Keywords: | Cyclic adenosine monophosphate Adenylate cyclase Sertoli cells Esox lucius (Teleostei) |
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