A new class of carriers that transport selective cargo from the trans Golgi network to the cell surface |
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| Authors: | Yuichi Wakana Josse van Galen Felix Meissner Margherita Scarpa Roman S Polishchuk Matthias Mann Vivek Malhotra |
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| Institution: | 1.Cell and Developmental Biology Programme, Centre for Genomic Regulation (CRG) and UPF, Barcelona, Spain;2.Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany;3.Telethon Institute of Genetics and Medicine, Naples, Italy;4.Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain |
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| Abstract: | We have isolated a membrane fraction enriched in a class of transport carriers that form at the trans Golgi network (TGN) and are destined for the cell surface in HeLa cells. Protein kinase D (PKD) is required for the biogenesis of these carriers that contain myosin II, Rab6a, Rab8a, and synaptotagmin II, as well as a number of secretory and plasma membrane‐specific cargoes. Our findings reveal a requirement for myosin II in the migration of these transport carriers but not in their biogenesis per se. Based on the cargo secreted by these carriers we have named them CARTS for CAR riers of the T GN to the cell S urface. Surprisingly, CARTS are distinct from the carriers that transport vesicular stomatitis virus (VSV)‐G protein and collagen I from the TGN to the cell surface. Altogether, the identification of CARTS provides a valuable means to understand TGN to cell surface traffic. |
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| Keywords: | CARTS Golgi membranes myosin II protein kinase D transport carriers |
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