Studies on the reaction of fluorescamine with primary amines |
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| Authors: | Silvano De Bernardo Manfred Weigele Voldemar Toome Karin Manhart Willy Leimgruber Peter Böhlen Stanley Stein Sidney Udenfriend |
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| Affiliation: | Chemical Research Department, Hoffmann-La Roche Inc., Nutley, New Jersey 07110 U.S.A.;Roche Institute of Molecular Biology, Nutley, New Jersey 07110 U.S.A. |
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| Abstract: | Fluorescamine is a useful reagent for the fluorometric assay of primary amines. The extent of the reaction between fluorescamine and primary amines, as well as the fluorescence intensities of the resulting fluorophors depend on pH, solvent composition and reagent concentration. Optimum values for these variables further depend on the amine under study. The influence of these parameters on the fluorogenic reaction of representative amines, and on their fluorophoric derivatives has been investigated, and the results are reported here. |
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| Keywords: | Authors to whom correspondence should be addressed. |
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