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Flavin binding by bacterial luciferase: Affinity chromatography of bacterial luciferase |
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| Authors: | C.A. Waters J.R. Murphy J.W. Hastings |
| Affiliation: | The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138 USA |
| Abstract: | Immobilized FMN covalently attached to Sepharose-6B-hexanoate binds bacterial luciferase. Immobilized flavin is also effective in its reduced form as a substrate in the light emitting reaction catalyzed by luciferase. |
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