Further studies on eukaryote DNA stimulation of amino acid incorporation in E. coli extracts |
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Authors: | Nando K Chatterjee De-Maw Chuang Herbert Weissbach |
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Institution: | Roche Institute of Molecular Biology, Nuttey, New Jersey 07110 USA |
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Abstract: | DNA from adenovirus-2 and mouse myeloma tumors stimulate RNA synthesis and amino acid incorporation into protein in a cell-free extract from Escherichia coli. The RNA synthesis is dependent on exogenous DNA, and the RNA can be hybridized to respective template DNA. A major part of this RNA is also found attached to E. coli polysomes suggesting that RNA with messenger-like activity has been synthesized. However, the in vitro-synthesized polypeptides using adenovirus DNA or myeloma DNA do not correspond in size or antigenic activity to either the virion proteins or immunoglobulins, respectively. |
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