The nucleolar protein SURF-6 is essential for viability in mouse NIH/3T3 cells |
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| Authors: | Mikhail Polzikov Charalambos Magoulas Olga Zatsepina |
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| Institution: | (1) Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 119997, Russia;(2) Institute of Cell and Molecular Science, Neuroscience Centre, Queen Mary, University of London, 4 Newark Street, London, E1 2AT, UK |
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| Abstract: | SURF-6 is a bona fide nucleolar protein comprising an evolutionary conserved family that extends from human to yeast. The
expression of the mammalian SURF-6 has been recently found to be regulated during the cell cycle. In order to determine the
importance of SURF-6 in mammalian cells, we applied the Tet-On system to regulate conditionally, in response to tetracycline,
the expression of an antisense RNA (asRNA) that targets Surf-6 mRNA in mouse NIH/3T3 cells. Induced Surf-6 asRNA caused an effective depletion of SURF-6 protein resulted in cell death and in an apparent arrest in the G1 phase of
the cell cycle. These results provide for the first time evidence that expression of SURF-6 is essential for mammalian cell
viability, and suggest that SURF-6 might participate in the progression of cell cycle. |
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| Keywords: | Nucleolus Nucleus SURF-6 Ribosomal biogenesis Cell cycle NIH/3T3 cells Tet-on |
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