Comparison of the synergistic action of two thermostable xylanases from GH families 10 and 11 with thermostable cellulases in lignocellulose hydrolysis |
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Authors: | Junhua Zhang Päivi TuomainenMatti Siika-aho Liisa Viikari |
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Institution: | a College of Forestry, Northwest A&F University, 3 Taicheng Road, Yangling 712100, China b VTT Technical Research Centre of Finland, P.O. Box 1000, FIN-02044 Espoo, Finland c Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 27, FIN-00014 Helsinki, Finland |
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Abstract: | Recombinant xylanase preparations from Nonomuraea flexuosa (Nf Xyn, GH11) and Thermoascus aurantiacus (Ta Xyn, GH10) were evaluated for their abilities to hydrolyze hydrothermally pretreated wheat straw. The GH family 10 enzyme Ta Xyn was clearly more efficient in solubilizing xylan from pretreated wheat straw. Improvement of the hydrolysis of hydrothermally pretreated wheat straw by addition of the thermostable xylanase preparations to thermostable cellulases was evaluated. Clear synergistic enhancement of hydrolysis of cellulose was observed when cellulases were supplemented even with a low amount of pure xylanases. Xylobiose was the main hydrolysis product from xylan. It was found that the hydrolysis of cellulose increased nearly linearly with xylan removal during the enzymatic hydrolysis. The results also showed that the xylanase preparation from T. aurantiacus, belonging to GH family 10 always showed better hydrolytic capacity of solubilizing xylan and acting synergistically with thermostable cellulases in the hydrolysis of hydrothermally pretreated wheat straw. |
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Keywords: | CBD cellulose binding domain DM dry matter DNS dinitrosalicylic acid GH glycosyl hydrolase HPAEC-PAD high performance anion exchange chromatography coupled with pulsed amperometric detection |
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