Molecular cloning of chicken prepro-orexin cDNA and preferential expression in the chicken hypothalamus |
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| Authors: | Ohkubo Takeshi Boswell Tim Lumineau Sophie |
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| Affiliation: | Center for Molecular Biology and Genetics, Mie University, Tsu, Japan. ohkubo@ag.kagawa-u.ac.jp |
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| Abstract: | Chicken prepro-orexin cDNA has been cloned, sequenced and characterized. The predicted amino acid sequence of chicken prepro-orexin cDNA revealed that orexin-A and -B are highly conserved among vertebrate species. In situ hybridization and immunohistochemistry localized orexin-positive cell bodies in the periventricular hypothalamic nucleus extending into the lateral hypothalamic area. Comparisons of orexin gene expression in the brains of 24-h-fasted and ad libitum-fed chickens were made using semi-quantitative RT-PCR. No significant differences in orexin mRNA expression were observed. |
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