Cell uptake of Zn(II)-phthalocyanine-containing liposomes by clathrin-mediated endocytosis |
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Authors: | Jorge Soriano Juan C Stockert Angeles Villanueva Magdalena Cañete |
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Institution: | 1. Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049, Madrid, Spain 2. Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, 28040, Madrid, Spain
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Abstract: | The study of uptake mechanisms of therapeutic drugs has a growing interest in biomedical research. In this work the cell uptake
and phototoxicity of the photosensitizer Zn(II)-phthalocyanine (ZnPc) in dipalmitoyl-phosphatidyl-choline liposomes have been
studied in the presence or absence of inhibitors of macropinocytosis (cytochalasin D), and clathrin-mediated endocytosis (dynasore).
No differences in the uptake or photodynamic damage were observed in A-549 cells subjected to incubation with either ZnPc
alone or in combination with cytochalasin D. On the contrary, co-incubation of A-549 cells with ZnPc and dynasore resulted
in a significant decrease of photodamage as well as negligible uptake of the photosensitizer. These results indicate that
ZnPc is internalized into cells preferentially by a mechanism of clathrin-mediated endocytosis. |
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