Tryptophan degradation in Saccharomyces cerevisiae: Characterization of two aromatic aminotransferases |
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Authors: | P Kradolfer P Niederberger R Hütter |
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Institution: | (1) Mikrobiologisches Institut, Eidg. Technische Hochschule, ETH-Zentrum, CH-8092 Zürich, Switzerland;(2) Present address: Migros-Fleischlabor, CH-1784 Courtepin, Switzerland |
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Abstract: | Tryptophan was found to be degraded in Saccharomyces cerevisiae mainly to tryptophol. Upon chromatography on DEAE-cellulose two aminotransferases were identified: Aromatic aminotransferase I was constitutively synthesized and was active in vitro with tryptophan, phenylalanine or tyrosine as amino donors and pyruvate, phenylpyruvate or 2-oxoglutarate as amino acceptors. The enzyme was six times less active with and had a twenty times lower affinity for tryptophan (K
m=6 mM) than phenylalanine or tyrosine. It was postulated thus that aromatic aminotransferase I is involved in vivo in the last step of tyrosine and phenylalanine biosynthesis. Aromatic aminotransferase II was inducible with tryptophan but also with the other two aromatic amino acids either alone or in combinations. With tryptophan as amino donor the enzyme was most active with phenylpyruvate and not active with 2-oxoglutarate as amino acceptor; its affinity for tryptophan was similar as for the other aromatic amino acids (K
m=0.2–0.4 mM). Aromatic aminotransferase II was postulated to be involved in vivo mainly in the degradation of tryptophan, but may play also a role in the degradation of the other aromatic amino acids.A mutant strain defective in the aromatic aminotransferase II (aat2) was isolated and its influence on tryptophan accumulation and pool was studied. In combination with mutations trp2
fbr, aro7 and cdr1-1, mutation aat2 led to a threefold increase of the tryptophan pool as compared to a strain with an intact aromatic aminotransferase II. |
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Keywords: | Saccharomyces cerevisiae Tryptophan degradation to tryptophol Degradation-defective mutant strain Aromatic aminotransferases Tryptophan accumulation |
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