| 平滑肌肌球蛋白B的超沉淀与肌球蛋白轻链磷酸化 |
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| 引用本文： | 陈明,李爱媛.平滑肌肌球蛋白B的超沉淀与肌球蛋白轻链磷酸化[J].中国生物化学与分子生物学报,1992,8(4):424-428. |
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| 作者姓名： | 陈明 李爱媛 |
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| 作者单位： | 中国科学院上海生理研究所，中国科学院上海生理研究所 上海 200031，上海 200031 |
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| 摘 要： | 本文报导了牛胃肌球蛋白B(天然肌动球蛋白)的超沉淀性质。当钙离子、钙调蛋白和ATP存在时,肌球蛋白B出现超沉淀,在pH6.8和7.5处,有两个峰值。Ca~(2+)(PCa值8-4)对超沉淀影响的浓度-反应曲线呈典型的S形,表明当Ca~(2+)浓度处于微摩尔水平时产生超沉淀。伴随超沉淀发生了肌球蛋白调节轻链磷酸化。这说明肌球蛋白轻链的Ca~(2+)-CaM依赖性磷酸化可能包含在脊椎动物平滑肌收缩活动的调节机制中。
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| 关 键 词： | 肌球蛋白B 超沉淀 肌球蛋白轻链 调节轻链磷酸化 聚丙烯酰胺凝胶电泳 |
| 收稿时间： | 1992-08-20 |
The Superprecipitation of Smooth Muscle Myosin B and Ca~(2+) CaM-Dependent Phosphorylation of the Myosin Light Chain |
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| Chen,Ming Li,Ai-yuan.The Superprecipitation of Smooth Muscle Myosin B and Ca~(2+) CaM-Dependent Phosphorylation of the Myosin Light Chain[J].Chinese Journal of Biochemistry and Molecular Biology,1992,8(4):424-428. |
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| Authors: | Chen Ming Li Ai-yuan |
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| Institution: | (Shanghai Institute of Physiology,Chinese Academy of Sciences,Shanghai 200031 |
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| Abstract: | The characteristics of superprecipitation of myosin B (natural actomyosin) from bovine stomach has been studied.It was found that the superprecipitation of myosin B occurs in the presence of calcium, calmodulin and ATP, showing two peaks at pH 6.8 and 7.5 The concentration-response curve for the effect of Ca2+(pCa values, from 8 to 4) on superprecipitation appears in typical S-form, indicating that myosin B superprecipitation occurs at Ca2+ concentration of micromolar level.The phosphorylation of myosin light chain is accompanied by superprecipitation.It is suggested that the Ca2+ -CaM-dependent phosphorylation of myosin light chain might be involved in the regulatory mechanism of contraction in vertebrate smooth muscle. |
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| Keywords: | Myosin B Superprecipitation Myosin light chain Phosphorylation of regulatory light chain Polyacrylamide gel electrophoresis |
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