Production of polygalacturonase byByssochlamys fulva |
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Authors: | P Kotzekidou |
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Institution: | (1) Food Science Division, Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, U.K.;(2) Present address: Department of Agricultural Industries-Food Science and Technology, Faculty of Agriculture, Aristotelian University of Thessaloniki, Box 250, 54006 Thessaloniki, Greece |
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Abstract: | Summary
Byssochlamys fulva was grown in two fermentation media using shake flasks, stirred fermentor and disc fermentor under conditions to give maximum production of pectolytic enzymes. Only polygalacturonase activity was detected in the culture filtrates during all fermentations. In all production conditions studied, no evidence of pectin methylesterase, pectin lyase, cellulase or proteinase activities were found. The maximum polygalacturonase activity (4.5 units/ml) was achieved when the microorganism was grown on medium II in shake flasks at pH 4.0–4.5 and 30°C after 12 days of fermentation. |
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Keywords: | Polygalacturonase Byssochlamys fulva Shake flask Stirred fermentor Disc fermentor |
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