Determination of 9α,11β-prostaglandin F2 in human urine and plasma by gas chromatography—mass spectrometry |
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Authors: | T Obata T Nagakura M Kammuri T Masaki K Maekawa K Yamashita |
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Abstract: | Sensitive and specific assay methods for 9α,11β-prostaglandin F2 (9α,11β-PGF2) by gas chromatography—mass spectrometry with electron impact ionization are described. The mass spectrometric assay for 9α,11β-PGF2 was based on the use of the methyl ester—dimethylisopropylsilyl ether derivative, and pentadeuterated PGF2α as a convenient internal standard. The calibration graph for 9α,11β-PGF2 was linear from 5 pg to 100 ng for both the standard and spiked biological samples. The limit of detection was 50 pg/ml for urine and 25 pg/ml for plasma (signal-to-noise RATIO = 2.3). The method was applied to the determination of 9α,11β-PGF2 in urine and plasma samples from patients with bronchial asthma. |
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