Isolation of myelin basic protein-reactive T-cell lines from normal human blood |
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| Authors: | J Burns A Rosenzweig B Zweiman R P Lisak |
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| Affiliation: | Department of Neurology, and Allergy and Immunology Section of the Department of Medicine, University of Pennsylvania School of Medicine, Multiple Sclerosis Research Center of the University of Pennsylvania-Wistar Institute, Philadelphia, Pennsylvania 19104 USA |
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| Abstract: | T-Cell lines which responded by proliferation to the autoantigen, myelin basic protein (MBP), were isolated from the blood of six of nine normal humans. These T-cell lines could be maintained in in vitro culture for up to 2 months through the use of Interleukin 2 and repeated MBP stimulation. Optimal antigen-induced proliferation required both antigen and antigen-presenting cells found in the adherent cell population of autologous peripheral blood mononuclear cells (PBM). The T-cell lines were predominantly of the helper phenotype (OKT3+, OKT4+, OKT8-) and responded to both human and guinea pig myelin basic protein. |
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| Keywords: | To whom correspondence should be addressed: Department of Neurology Hospital of the University of Pennsylvania 3400 Spruce St. Philadelphia Pa. 19104. |
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