A novel approach to the establishment of dual cultures of pearl millet and Sclerospora graminicola |
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Authors: | Geetha Upadhyaya M B Shivanna H S Prakash H S Shetty |
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Institution: | (1) Department of Studies in Applied Botany, University of Mysore, Manasagangotri, 570 006 Mysore, India |
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Abstract: | Dual cultures were successfully established using malformed florets of pearl millet infected with Sclerospora graminicola, the downy mildew pathogen. A higher proportion (86%) of calli from malformed florets formed dual cultures on Murashige and Skoog's (MS) medium with 2 mg 1-1 of 2,4-dichlorophenoxy acetic acid (2,4-d), compared to shoot tips (25%). Fungal mycelium covered the entire surface of the callus within 30 days of placement of explants on the MS medium with 2 mg 1-1 of 2,4-d. The infected calli also differentiated and produced plantlets when transferred to MS medium without 2,4-d. |
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Keywords: | dual culture malformed floret pearl millet Sclerospora graminicola |
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