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| 激素处理及非生物胁迫下枳PtPPF-1基因的表达特性 | |
| 引用本文： | 罗海澜,王飞,罗海淑,李新伟,夏仁学. 激素处理及非生物胁迫下枳PtPPF-1基因的表达特性[J]. 西北植物学报, 2011, 31(10) |
| 作者姓名： | 罗海澜 王飞 罗海淑 李新伟 夏仁学 |
| 作者单位： | 1. 漯河医学高等专科学校,河南漯河,462002 2. 江西省吉水县乌江农技站,江西吉水,331600 3. 华中农业大学园艺林学学院,湖北武汉,430070 |
| 基金项目： | 国家科技部促进三峡移民开发专项(2004EP090019) |
| 摘 要： | PtPPF-1是从枳叶片中分离出的与豌豆中的PPF-1具有很高同源性的基因。PPF-1基因能通过控制叶绿体发育而延缓叶片衰老,同时PPF-1能被外源GA3诱导,是与植物营养生长密切相关的基因。本研究以实生苗枳[Poncirus trifoliata(L.)Raf.]为材料,采用半定量RT-PCR对不同激素处理及非生物胁迫(低温、干旱、盐)下枳叶中PtPPF-1的表达情况进行了分析,以期明确PtPPF-1的功能。结果表明,GA3I、AA、ABA促进了PtPPF-1的表达,KT对PtPPF-1的表达有抑制作用;低温、干旱、盐胁迫能诱导PtPPF-1的表达。推测GA3、IAA、ABA信号通路可能与KT信号通路作用于PtPPF-1的表达存在不同的机制。 |
| 关 键 词： | 枳 PtPPF-1 激素 非生物胁迫 RT-PCR |
PtPPF-1 Expression Properties under Phytohormone and Abiotic Stress Treatments in Poncirus trifoliata(L.) Raf. |
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| LUO Hai-lan,WANG Fei,LUO Hai-shu,LI Xin-wei,XIA Ren-xue. PtPPF-1 Expression Properties under Phytohormone and Abiotic Stress Treatments in Poncirus trifoliata(L.) Raf.[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(10) | |
| Authors: | LUO Hai-lan WANG Fei LUO Hai-shu LI Xin-wei XIA Ren-xue |
| Affiliation: | LUO Hai-lan1,WANG Fei1,LUO Hai-shu2,LI Xin-wei1,XIA Ren-xue3*(1 Luohe Medical College,Luohe,Henan 462002,China,2 Jiangxi Jishui Wujiang Agrotechnical Station,Jishui,Jiangxi 331600,3 Horticulture and Forestry College of Huazhong Agricultural University,Wuhan,Hubei 430070,China) |
| Abstract: | PtPPF-1 was cloned from leaf of Poncirus trifoliata(L.) Raf.,showed high similarity with PPF-1 from pea.PPF-1 could delay senescence of leaves by modulating the development of chloroplast.While the gene could be provoked by exogenous GA3,showing close relative with plant growth.In this study,the expression properties of PtPPF-1 were primarily studied after treatment by several phytohormone(GA3,IAA,ABA and KT) in P.trifoliata seedlings.The expression of PtPPF-1 in leaves was increased when treated by GA3,IAA... |
| Keywords: | Poncirus trifoliata(L.) Raf. PtPPF-1 phytohormone abiotic stress RT-PCR |
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