| 转萝卜过氧化物酶基因毕赤酵母株系培养条件的优化 |
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| 引用本文： | 王一帆,李芳军,杨静,王丽,王林嵩. 转萝卜过氧化物酶基因毕赤酵母株系培养条件的优化[J]. 生物技术通报, 2010, 0(4) |
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| 作者姓名： | 王一帆 李芳军 杨静 王丽 王林嵩 |
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| 作者单位： | 河南师范大学生命科学学院,新乡,453007 |
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| 基金项目： | 国家自然科学基金项目，国家大学生创新性实验计划项目，河南师范大学大学生创新性实验计划项目，河南师范大学(国家级)实验教学示范中心大学生创新性实验计划项目 |
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| 摘 要： | 为了提高转萝卜过氧化物酶基因RsPrx1毕赤酵母株系的表达产量,对培养条件进行优化。试验结果表明,26℃,pH7.4,甲醇浓度0.5%,通气面积1.5cm2,225r/min条件下,萝卜过氧化物酶RsPrx1表达量达50.95U/mL。在优化条件下,对培养基补充方式和菌株的遗传稳定性研究表明,彻底更换培养基较补充培养基提高3倍得率,转基因菌株遗传稳定性较高。
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| 关 键 词： | 萝卜过氧化物酶 毕赤酵母 转基因 培养条件 |
The Optimum Cultivation for Improved Heterologous Expression of Radish Peroxidase in Pichia pastoris |
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| Wang Yifang,Li Fangjun,Yang Jing,Wang Li,Wang Linsong. The Optimum Cultivation for Improved Heterologous Expression of Radish Peroxidase in Pichia pastoris[J]. Biotechnology Bulletin, 2010, 0(4) |
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| Authors: | Wang Yifang Li Fangjun Yang Jing Wang Li Wang Linsong |
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| Affiliation: | Wang Yifang Li Fangjun Yang Jing Wang Li Wang Linsong(College of Life Science,Henan Normal University,Xinxiang 453007) |
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| Abstract: | The optimum cultivation have been investigated for improved heterologous expression of RsPrx1(a peroxidase of radish)in Pichia pastoris.The results showed that the expression of RsPrx1 could reached 50.95 U/mL in 26℃, pH7.4, 0.5% methanol, 1.5 cm~2 aeration area, 225 r/min.In the optimum cultivation, the total activity of peroxidase was about 3 times higher in absolutely replaced medium continuous culture than that partly added medium continuous culture.The PCR results showed the transgenic strain was hereditary stability. |
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| Keywords: | Radish peroxidase Pichia pastoris Transgene Culture condition |
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