Vertical fluctuations of phospholipid acyl chains in bilayers |
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| Authors: | J R Wardlaw W H Sawyer K P Ghiggino |
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| Institution: | Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia. |
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| Abstract: | The possibility of vertical displacement of acyl chains in lipid bilayers has been examined by quenching the fluorescence of 2-(9-anthroyloxy)palmitic acid with 5- and 16-doxylstearates in dipalmitoylphosphatidylcholine unilamellar vesicles. Measurement of lifetime and steady-state quenching showed that the dynamic component of quenching was independent of the transverse position of the quencher indicating that a quencher at the 16-position could interact with a fluorophore at the 2-position with high frequency. The differences in steady-state quenching could be accounted for by the differences in the static component of quenching. The results provide further evidence for rapid vertical displacements of acyl chains in phospholipid bilayers. |
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