A simplified method for the purification of riboflavin-binding protein from hen's egg yolk |
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| Authors: | U S Murthy K Sreekrishna P R Adiga |
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| Institution: | Department of Biochemistry, Indian Institute of Science, Bangalore-360012, India |
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| Abstract: | A simple and efficient procedure for the purification of the riboflavin-binding protein from hen's egg yolk is described. This method involves the removal by exclusion of lipoproteins and subsequent fractionation of soluble yolk proteins held on a DEAE-cellulose column by a salt gradient which is followed by purification by gel filtration on Sephadex G-100. The protein thus isolated is homogeneous by various physicoehemical, immunological, and functional criteria. |
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