Correlation between the induction of a gene for Δ1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress |
| |
| Authors: | Yoshu Yoshiba Tomohiro Kiyosue Takeshi Katagiri Hiroko Ueda Tsuyoshi Mizoguchi Kazuko Yamaguchi-Shinozaki Keishiro Wada Yoshinori Harada Kazuo Shinozaki |
| |
| Institution: | Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, Ibaraki 305, Japan;Advanced Research Laboratory, Hitachi Ltd., Hatoyama, Saitama 350-03, Japan;Institute of Biologial Science, Tsukuba University, Tsukuba, Ibaraki 305, Japan;Department of Biology, Faculty of Science, Kanazawa University, Kanazawa, Ishikawa 920-11, Japan;Japan International Research Center of Agricultural Science, Tsukuba, Ibaraki 305, Japan |
| |
| Abstract: | The isolation and characterization is reported of a cDNA for Δ1-pyrroline-5-carboxylate (P5C) synthetase (cAtP5CS), an enzyme involved in the biosynthesis of proline, from a cDNA library prepared from a dehydrated rosette plant of Arabidopsis thaliana . Southern blot analysis suggested that only one copy of the corresponding gene ( AtP5CS ) is present in A. thaliana . The deduced amino acid sequence of the P5CS protein (AtP5CS) from A. thaliana exhibited 74% homology to that of the P5CS from Vigna aconitifolia . Northern blot analysis revealed that the gene for P5CS was induced by dehydration, high salt and treatment with ABA, while it was not induced by heat or cold treatment. Moreover, the simultaneous accumulation of proline was observed as a result of the former treatments in A. thaliana . A cDNA for P5C reductase (cAtP5CR) was also isolated from A. thaliana and Northern blot analysis was performed. The AtP5CR gene was not induced to a significant extent by dehydration or high-salt stress. These observations suggest that the AtP5CS gene plays a principal role in the biosynthesis of proline in A. thaliana under osmotic stress. |
| |
| Keywords: | |
|
|
