Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat |
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Authors: | Robert Olivier Abelard Christine Dedryver Françoise |
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Institution: | (1) INRA, Station d'Amélioration des Plantes, Laboratoire Céréales, BP 29, 35653 Le Rheu Cédex, France |
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Abstract: | The Yr17 gene, which is present in many European wheat cultivars, displays yellow rust resistance at the seedling stage. The
gene introduced into chromosome 2A from Aegilops ventricosa was previously found to be closely linked (0.5 cM) to leaf and
stem rust resistance genes Lr37 and Sr38, respectively. The objective of this study was to identify molecular markers linked
to the Yr17 gene. We screened with RAPD primers, for polymorphism, the DNAs of cv. Thatcher and the leaf rust-resistant near-isogenic
line (NIL) RL 6081 of cv. Thatcher carrying the Lr37 gene. Using a F2 progeny of the cross between VPM1 (resistant) and Thésée
(susceptible), the RAPD marker OP-Y15580 was found to be closely linked to the Yr17 gene. We converted the OP- Y15580 RAPD
marker into a sequence characterized amplified region (SCAR). This SCAR marker (SC-Y15) was linked at 0.8 ± 0.7 cM to the
Yr17 resistance gene. We tested the SC-Y15 marker over a survey of 37 wheat cultivars in order to verify its consistency in
different genetic backgrounds and to explain the resistance of some cultivars against yellow rust. Moreover, we showed that
the Xpsr150-2Mv locus marker of Lr gene described by Bonhomme et al. 6] which possesses A. ventricosa introgression on the
2A chromosome was also closely linked to the Yr17 gene. Both the SCAR SC-Y15 and Xpsr150-2Mv markers should be used in breeding
programmes in order to detect the cluster of the three genes Yr17, Lr37 and Sr38 in cross progenies.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | wheat DNA markers yellow rust resistance Yr17 |
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