烟粉虱内共生菌16S rDNA的变异与系统发生

对 5年连续饲养在不同种寄主植物上的B型烟粉虱北京种群的内共生菌 1 6SrDNA基因进行了PCR扩增和测序。结合已知序列 ,构建了不同寄主植物烟粉虱初生内共生菌约 1 0 0 0bp的 1 6SrDNA及次生内共生菌约1 2 5 0bp的 1 6SrDNA的分子系统树。结果表明 ,中国北京不同寄主植物的B型烟粉虱内共生菌及世界其它地区烟粉虱内共生菌可能是同一种的不同生态型 ,内共生菌在其宿主分化后进行了选择 ,之后与其宿主长期共同进化、共同适应 ,为宿主对不同生境的适应提供了一定的基础

Phylogenetic Relationships and Evolution of Endosymbionts in Bemisia tabaci Based on Partial 16S rDNA Sequences

Endosymbionts evolute with insects and benefit from each other. Endosymbiont affects the growth, fertilizing and transmitting disease of insect. In order to understand the control methods and evolution mechanism of Bemisia tabaci , cloning and sequencing of 16S rDNA of endosymbionts in Bemisia tabaci long term living on different host plants in Peking were conducted. Molecular phylogenetic trees, including about 1000bp 16S rDNA of primary endosymbionts in Bemisia tabaci and about 1250bp16S rDNA of secondary endosymbionts in Bemisia tabaci ,were reconstructed. The results showed that primary endosymbionts in Bemisia tabaci were Proteobacteria,Gammaproteobacteria,Oceanospirillales,Halomonadaceae,Zymobacter group, Candidatus Portiera aleyrodidarum, and secondary endosymbionts in Bemisia tabaci were Proteobacteria,Gammaproteobacteria,Enterobacteriales, Enterobacteriaceae. The endosymbionts from Bemisia tabaci of different host plants and other countries might belong to different ecological types of one species. The conclusion could be drawn that there are independent acquisition of various endosymbionts in Bemisia tabaci from different host plants, and endosymbionts play important roles on Bemisia tabaci accommodation with different environment.