Seasonal culture of dormant reproductive buds ofSalix tetrasperma: Analysis of the flowering process |
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Authors: | Rajiv Angrish K K Nanda |
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Institution: | (1) Botany Department, Panjab University, 160014 Chandigarh, India |
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Abstract: | The flowering process in a female tree ofSalix tetrasperma was analysed by culturing its reproductive buds at different developmental stages during the dormant period on a chemically
defined medium and examining the nature of sprouts produced by them. Buds at the upper eight nodes of the actively growing
shoots developing in an acropetal sequence were cultured in separate lots. While all the buds collected from the 1st and 2nd
nodes of the branches from the top downwards were vegetative and produced shoots, a considerable number of those collected
from the 3rd and 4th nodes were reproductively determined and produced catkins. All the buds obtained from the 5th node and
below were reproductive.
Reproductive buds were cultured at regular time intervals during the dormant period. Freshly formed buds cultured in March
during the spring growth flush produced catkins and were therefore reproductively determined. However, such a determination
was not tantamount to flowering, as the floral meristems present in the axils of catkin bracts remained quiescent. Floral
meristems of the buds cultured during April to August developed into small vegetative shoots. This was followed by the crucial
period during September to December when the hitherto vegetative sprouts of the floral meristems showed a gradual transition
into ovaries (female flowers) resulting in fertile catkins. Catkins produced from buds cultured in January and February produced
well-developed ovaries. |
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Keywords: | Salix dormant reproductive buds in vitro culture flowering process |
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