丙酮酸钠对失血性休克大鼠缺血/再灌注损伤的保护作用

目的:研究丙酮酸钠对失血性休克后缺血/再灌注损伤的保护作用。方法:制作失血性休克大鼠模型,回输全血,同时分别给予生理盐水、谷胱甘肽和丙酮酸钠适量,于再灌注后3h处死动物。检测血浆乳酸脱氢酶(LDH)和谷草转氨酶(GOT)的活性、组织丙二醛(MDA)的含量和髓过氧化物酶(MPO)的活性,观察心、肝、肺和肾组织的病理变化。结果:丙酮酸钠组与生理盐水组相比,血浆LDH和GOT的活性降低,肝、肺和肾组织MDA的含量下降,心、肺和肾组织MPO活性降低,效果优于谷胱甘肽。心、肝、肺和肾组织形态学观察显示,丙酮酸钠使组织损伤减轻。结论:丙酮酸钠对失血性休克后再灌注损伤具有保护作用。其作用机制可能与清除氧自由基、减少中性粒细胞的浸润、减轻炎性反应有关。

Protective effect of sodium pyruvate on ischemia/reperfusion injury of rats subjected to hemorrhagic shock

Aim:To study the protective effect of sodium pyruvate on ischemia/reperfusion injury following hemorrhagic shock.Methods:Rat models of hemorrhagic shock were built up.When the shed blood was infused,the rats were also randomly provided by one of normal saline,glutathione and sodium pyruvate.Rats were killed 3 hours after the reperfusion,the activity of plasma lactate dehydrogenase(LDH)and glutamic-oxaloacetic transaminase(GOT),the level of tissue malondialdehyde(MDA)and the activity of tissue myeloperoxidase(MPO)were detected.Biopsy specimens were obtained to investigate morphological changes of the myocardial,hepatic,lung and renal tissue.Results:The activity of plasma LDH and GOT,the level of MDA of hepatic,lung and renal tissue and the activity of MPO of myocardial,lung and renal tissue decreased remarkably in group given sodium pyruvate compared with group given normal saline,and the effect of group given sodium pyruvate was more remarkable than group given glutathione.Conclusion:These data support the view that sodium pyruvate shows protective effect on ischemia/reperfusion injury following hemorrhagic shock.It is possibly relevant to scavenging of oxygen free radicals,reduction of neutrophil,and anti-inflammatory response.