Transglycosylation of Stevioside by Cyclodextrin Glucanotransferases of Various Groups of Microorganisms |
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| Authors: | V A Abelyan A M Balayan V T Ghochikyan A A Markosyan |
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| Institution: | (1) Institute of Microbiology, National Academy of Sciences of Armenia, Abovyan, 375510, Armenia;(2) Stevian Biotechnological Corporation, Kuala Lumpur, 50450, Malaysia |
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| Abstract: | Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside (in order to remove bitterness and aftertaste), with cyclodextrins (CDs) being used as donors. It was shown that CGTases produced by extremophilic microorganisms are effective biocatalysts. Optimum temperature and pH of these enzymes were 45°C and pH 6.5–7.5, respectively. The optimum stevioside-to-CD ratio and total concentration of dry matter for the synthesis of the best-tasting product were 1 : 1 (w/w) and 11.6%, respectively. |
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