RFLP genetic linkage maps from four F(2.3) populations and a joinmap of Gossypium hirsutum L |
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Authors: | Ulloa M Meredith W R Shappley Z W Kahler A L |
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Institution: | (1) USDA-ARS, Crop Genetics and Production Research Unit, Stoneville, MS 38776, USA e-mail: mulloa@ars.usda.gov Tel.: +1-662/686-5464, Fax: +1-662/686-5218, US;(2) Monsanto, 700 Chesterfield Parkway N. GG6A, St. Louis, MO 63198, USA, US;(3) Biogenetic Services Incorporated, 801 32nd Avenue, Brookings, SD 57006, USA, US |
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Abstract: | An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton (Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from
199 bulk-sampled plots of an F2.3 (HQ95–6×’MD51ne’) population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers
of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from
155 bulk-sampled plots of an F2.3 (119– 5 sub-okra×’MD51ne’) population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between
markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between
populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284
loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately
31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged
in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships,
gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage
joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain).
Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits
on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).
Received: 23 February 2001 / Accepted: 8 June 2001 |
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Keywords: | Gossypium hirsutum L RFLP Restriction fragment length polymorphism Genetic map Linkage Upland cotton Delta cotton Texas high plain cotton |
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