Lipid extracts from different algal species:1H and13C-NMR spectroscopic studies as a new tool to screen differences in the composition of fatty acids,sterols and carotenoids |
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| Authors: | Piero Pollesello Renato Toffanin Erminio Murano Sergio Paoletti Roberto Rizzo Bjarne J. Kvam |
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| Affiliation: | (1) Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, via A. Valerio 38, I-34127, Trieste, Italy;(2) POLY-biòs Research Center, LBT, Area di Ricerca di Trieste, Padriciano, 99, I-34012 Trieste, Italy |
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| Abstract: | One- and two-dimensional1H- and13C-NMR spectra of lipid extracts fromUlva rigida, Gracilaria longa, Fucus virsoides andCodium tomentosum collected in the northern Adriatic Sea allowed screening of the content of fatty acid chains, carotenoids, free and acylated cholesterol and chlorophylls. The carotenoid-to-polyunsaturated fatty acid molar ratio was taken as a comparison parameter in samples ofUlva rigida collected in differentloci and seasons; the value was markedly higher in samples from the Lagoon of Venice than from marine coastal waters. The total cholesterol concentration was evaluated by1H-NMR spectroscopy and similar values were found for all species. Two-dimensional heterocorrelated NMR spectroscopy was shown to give characteristic fingerprints of the lipid extracts from algal samples as regards the content in chlorophylls, unsaturated fatty acids and carotenoids.author for correspondence |
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| Keywords: | lipid extract 1H-NMR 13C-NMR carotenoids polyunsaturated fatty acids Ulva rigida Gracilaria longa Fucus virsoides Codium tomentosum |
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