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Ethanol production by Saccharomyces cerevisiae using lignocellulosic hydrolysate from Chrysanthemum waste degradation
Authors:Balkys Quevedo-Hidalgo  Felipe Monsalve-Marín  Paulo César Narváez-Rincón  Aura Marina Pedroza-Rodríguez  Mario Enrique Velásquez-Lozano
Institution:1. Grupo de Biotecnología Ambiental e Industrial (GBAI), Laboratorio de Biotecnología Aplicada, Departamento de Microbiología, Pontificia Universidad Javeriana, Bogotá, Colombia
2. Carrera 7 No 43-68 oficina 618, edificio 52, Bogotá, Colombia
3. Industrial Microbiologist, Pontificia Universidad Javeriana, Bogotá, Colombia
4. Grupo de Procesos Químicos y Bioquímicos, Laboratorio de Ingeniería Química, Departamento de Ingeniería Química y Ambiental, Universidad Nacional de Colombia sede Bogotá, Carrera 30 No 45-03, edificio 412, Bogotá, Colombia
5. Grupo de Biotecnología Ambiental e Industrial (GBAI), Laboratorio de Microbiología ambiental, Departamento de Microbiología, Pontificia Universidad Javeriana, Carrera 7 No 43-68 oficina 618, edificio 52, Bogotá, Colombia
Abstract:Ethanol production derived from Saccharomyces cerevisiae fermentation of a hydrolysate from floriculture waste degradation was studied. The hydrolysate was produced from Chrysanthemum (Dendranthema grandiflora) waste degradation by Pleurotus ostreatus and characterized to determine the presence of compounds that may inhibit fermentation. The products of hydrolysis confirmed by HPLC were cellobiose, glucose, xylose and mannose. The hydrolysate was fermented by S. cerevisiae, and concentrations of biomass, ethanol, and glucose were determined as a function of time. Results were compared to YGC modified medium (yeast extract, glucose and chloramphenicol) fermentation. Ethanol yield was 0.45 g g?1, 88 % of the maximal theoretical value. Crysanthemum waste hydrolysate was suitable for ethanol production, containing glucose and mannose with adequate nutrients for S. cerevisiae fermentation and low fermentation inhibitor levels.
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