Effects of human alpha-interferon on granulocyte-macrophage progenitor cells (CFU-GM) in vitro |
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| Authors: | J S Schwamborn R M Nickels B D Katthagen H W Pees P G Scheurlen |
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| Institution: | Medizinische Klinik I, Universit?ts- und Polikliniken, Homburg/Saar, Federal Republic of Germany. |
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| Abstract: | Two preparations of human interferon (IFN)-alpha were assessed for their influence on granulocyte-macrophage progenitor cells (CFU-GM) in vitro. Both highly purified human IFN-alpha Ly and recombinant IFN-alpha 2a suppressed CFU-GM colony formation in a dose-dependent manner using low-density bone-marrow target cells. Suppression of CFU-GM colony formation was accompanied by an increase in clusters. However, depletion of monocytes, T lymphocytes and B lymphocytes from low-density bone-marrow cells resulted in insensitivity of progenitor cells to IFN-alpha. These results demonstrate that the effects of human IFN-alpha on myeloid progenitor cells (CFU-GM) are mediated by accessory cells within the bone marrow. |
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