Quantitative studies on prostaglandin synthesis in man. II. Determination of the major urinary metabolite of prostaglandins F1 alpha and F2 alpha |
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Authors: | M Hamberg |
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Affiliation: | Department of Chemistry, Karolinska Institutet, S10401, Stockholm, Sweden |
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Abstract: | A method was developed for quantitative determination of 5α,7α-dihydroxy-11-ketotetranor-prostane-1,16-dioic acid, the major urinary metabolite of prostaglandins F1α and F2α in man. The method was based on the use of the O-methyloxime derivative of [5β-3H; 10,10,12,12-2H4]5α,7α-dihydroxy-11-ketotetranor-prostane-1,16-dioic acid as internal standard and determination of ratios between unlabeled and deuterium-labeled molecules by multiple-ion analysis. Excretion values found for healthy human subjects were: males, (n = 10, mean value, 24.0 ± 17.2 (SD) μg) and females, (n = 10, mean value, 10.5 ± 2.1 (SD) μg). |
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