Reconstitution of Photosystem Ⅱ Reaction Center with Cu-ChlorophyⅡ a |
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作者姓名: | Shuang Liu Feng-Qin Dong Chun-Hong Yang Chong-Qin Tang Ting-Yun Kuang |
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作者单位: | Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China |
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基金项目: | The authors are grateful to Professor Yan-Dao Gong (Tsinghua University, Beijing, China) for help with the CD spectra experiment. |
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摘 要: | An Isolated photosystem (PS) II reaction center (RC) with altered pigment content was obtained by chemical exchange of native chlorophyll a (Chl) with externally added Cu-Chl a (Cu-Chl). Pigment composition and spectroscopic properties of the RC exchanged with Cu-Chl were compared with native RC and RC treated with Chl In the same way. High-performance liquid chromatography analysis showed approximately 0.5 Cu-Chl per two pheophytln in the Cu-Chl-reconstltuted RC preparation. Insertion of Cu-Chl resulted in a decrease In absorption at 670 nm and an Increase at 660 nm, suggesting that the peripheral Chl may have been displaced. Fluorescence emission spectra of the Cu-Chl-reconstituted RC displayed a marked decrease In fluorescence yield and a blue shift of the band maximum, accompanied by the appearance of a broad peak at a shorter wavelength, Indicating that energy transfer In the modified RC was disturbed by Cu-Chl, a quencher of the excited state. However, there were few differences in the circular dichrolsm (CD) spectra, suggesting that the arrangement of pigments and proteins responsible for the CD signal was not significantly affected. In addition, no obvious change In peptlde components was found after the exchange procedure.
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关 键 词: | 光合体系 基因重组 植物 生物 |
收稿时间: | 2005-11-17 |
修稿时间: | 2005-11-172006-03-27 |
Reconstitution of Photosystem II Reaction Center with Cu-Chlorophyll a |
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Authors: | Shuang Liu Feng-Qin Dong Chun-Hong Yang Chong-Qin Tang Ting-Yun Kuang |
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Institution: | Institute of Botany, the Chinese Academy of Sciences;, Beijing 100093, China) |
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Abstract: | An isolated photosystem (PS) II reaction center (RC) with altered pigment content was obtained by chemical exchange of native chlorophyll a (Chl) with externally added Cu‐Chl a (Cu‐Chl). Pigment composition and spectroscopic properties of the RC exchanged with Cu‐Chl were compared with native RC and RC treated with Chl in the same way. High‐performance liquid chromatography analysis showed approximately 0.5 Cu‐Chl per two pheophytin in the Cu‐Chl‐reconstituted RC preparation. Insertion of Cu‐Chl resulted in a decrease in absorption at 670 nm and an increase at 660 nm, suggesting that the peripheral Chl may have been displaced. Fluorescence emission spectra of the Cu‐Chl‐reconstituted RC displayed a marked decrease in fluorescence yield and a blue shift of the band maximum, accompanied by the appearance of a broad peak at a shorter wavelength, indicating that energy transfer in the modified RC was disturbed by Cu‐Chl, a quencher of the excited state. However, there were few differences in the circular dichroism (CD) spectra, suggesting that the arrangement of pigments and proteins responsible for the CD signal was not significantly affected. In addition, no obvious change in peptide components was found after the exchange procedure. (Managing editor: Ping He) |
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Keywords: | Cu-chlorophyll a photosystem II reaction center reconstitution |
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