Metabolic transformation of 14C-glucose into tissue proteins of Ancylostoma caninum |
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| Authors: | M E Pérez Giménez A Giménez K Gaede |
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| Affiliation: | 1. SALUVET group, Animal Health Department, Faculty of Veterinary Sciences, Complutense University of Madrid, Ciudad Universitaria s/n, Madrid 28040, Spain;2. Interdisciplinary Laboratory of Clinical Analysis, Interlab-UMU, University of Murcia, Murcia, Espinardo 30100, Spain;3. Animal Health Department, University of Murcia, Murcia, Espinardo 30100, Spain;4. Department of Biochemistry and Molecular Biology-A, University of Murcia, Murcia, Espinardo 30100, Spain;5. Statistical Support Section (SAE), Scientific and Research Area (ACTI), University of Murcia, Murcia, Espinardo 30100, Spain;1. Service d’anesthésie-réanimation, Hôpital des forces de sécurité Intérieure de La Marsa, 29, rue Tahar Ben Achour, 2070 La Marsa, Tunisie; Faculté de médecine de Tunis, Université El Manar, rue Djebal Lakhdar, 1006 Tunis, Tunisie;2. Pôle anesthésie-réanimation et Département des études paramédicales, Faculté de médecine, Université Côte d’Azur, 28, avenue Valombrose, 06107 Nice cedex 2, France;1. General Hospital “Sava Surgery”, Kej 29 decembra 2, Niš, Serbia;2. Plastic and Reconstructive Surgery Clinic, Clinical Center Niš, Niš, Serbia;3. Department of Medical Statistics and Informatics, Faculty of Medicine, University of Niš, Niš, Serbia;4. Department of Microbiology and Immunology, Faculty of Medicine, University of Niš, Niš, Serbia |
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| Abstract: | ![]()
A hookworm seems to feed on the blood of the host. This has been demonstrated fairly conclusively for the canine hookworm, Ancylostoma caninum. However, when hookworms are incubated in an artifical medium they are feeding also on these solutions and will ingest dissolved isotopically labeled compounds. During the present study 14C-glucose was taken up, actively metabolized by A. caninum, and partially transformed into amino acids and proteins of the parasite. About 20% of the amount of 14C-glucose (U.L.) present in one ml of the incubation medium was ingested by 130–165 worms of an average individual weight of 0.37–1.3 mg during a period of 4 hours. Of this activity, 70% was found in the soluble fraction of the homogenate and 0.69% of the glucose taken up had been incorporated into the soluble proteins. The utilization of glucose carbon atoms for amino acid synthesis by A. caninum is accomplished by common biochemical reactions: reductive animation and transamination. Aminating enzymes, such as glutamate dehydrogenase and glutamic-pyruvic transaminase, have been shown to be present in homogenates of the worm tissue. |
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