Chemically defined conditions for human iPSC derivation and culture |
| |
Authors: | Chen Guokai Gulbranson Daniel R Hou Zhonggang Bolin Jennifer M Ruotti Victor Probasco Mitchell D Smuga-Otto Kimberly Howden Sara E Diol Nicole R Propson Nicholas E Wagner Ryan Lee Garrett O Antosiewicz-Bourget Jessica Teng Joyce M C Thomson James A |
| |
Institution: | Morgridge Institute for Research, Madison, Wisconsin, USA. |
| |
Abstract: | We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|