High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins |
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| Authors: | Anna Zawadzka-Kazimierczuk Wiktor Koźmiński Hana Šanderová Libor Krásný |
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| Affiliation: | 1.Faculty of Chemistry,University of Warsaw,Warsaw,Poland;2.Laboratory of Molecular Genetics of Bacteria, Department of Bacteriology,Institute of Microbiology, Academy of Sciences of the Czech Republic,Prague,Czech Republic |
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| Abstract: | ![]()
Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences. |
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