Chemotaxonomic characterization of rice seedling blight complex using fatty acid methyl ester (FAME) profiles |
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Authors: | Seint San Aye Shinji Fukuda Masaru Matsumoto |
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Institution: | (1) Institute of Tropical Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan |
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Abstract: | Rice seedling blight is an important disease caused by a complex of fungi that include Fusarium, Rhizopus, Pythium, and Trichoderma species. A modified MIDI method was used for extraction of fatty acids from these causal pathogens, and fatty acid methyl
ester (FAME) profiles were characterized. Factors that might affect fatty acid production, such as period of culture and saponification
in extraction, were also evaluated. A total of 14 fatty acids were detected, and FAME profiles showed quantitative and qualitative
variations by discriminant analysis and principal component analysis. Genus-specific FAME profiles consisting of the types
of fatty acid produced and remarkable components of individual fatty acids were observed. The possibility of application as
chemotaxonomic methods based on the FAME profiles for diagnosis of the rice seedling blight complex is also discussed. |
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Keywords: | Fatty acid methyl ester Modified MIDI method Rice seedling blight |
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