Flow Cytometry of Mitotic Cells |
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| Authors: | H.J. Anderson G. de Jong I. Vincent M. Roberge |
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| Affiliation: | aDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada;bDivision of Medical Oncology, British Columbia Cancer Agency, Vancouver, British Columbia, Canada;cDepartment of Pathology, Albert Einstein College of Medicine of Yeshiva University, New York, New York, 10461 |
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| Abstract: | We have developed a procedure using flow cytometric measurement of a mitosis-specific antigen that may be used to count mitotic cells and sort them from nonmitotic cells. The procedure may also be used in conjunction with measurement of cellular DNA content and of bromodeoxyuridine incorporation into cellular DNA to assign cells to the G1/G0, S, G2, or M phase of the cell cycle. |
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| Keywords: | cell cycle/mitosis monoclonal antibody flow cytometry cell sorting |
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