1,3-Propandiol production by engineered Hansenula polymorpha expressing dha genes from Klebsiella pneumoniae |
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| Authors: | Won-Kyung Hong Chul-Ho Kim Sun-Yeon Heo Lian Hua Luo Baek-Rock Oh Dina Rairakhwada Jeong-Woo Seo |
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| Institution: | (1) Microbe-based Fusion Technology Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk, 580-185, South Korea;(2) Research Center of Bioactive Materials, Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, Chonbuk, 561-756, South Korea;(3) School of Biological Science and Technology, Chonnam National University, Gwangju, 500-757, South Korea; |
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| Abstract: | Currently, 1,3-propanediol (1,3-PD) is an important chemical widely used in polymer production, but its availability is being
restricted owing to its expensive chemical synthesis. A methylotrophic yeast Hansenula polymorpha was engineered by expression of dhaB1, dhaB2, dhaB3, dhaB
RA1
and dhaB
RA2
encoding glycerol dehydratase complex and dhaT encoding 1,3-PD oxidoreductase from Klebsiella pneumoniae under direction of promoter of glyceraldehyde-3 phosphate dehydrogenase (GAPDH). The engineered recombinant yeast strain
can produce 1,3-PD from glucose (2.4 g L−1) as well as glycerol (0.8 g L−1), which might lead to a safe and cost-effective method for industrial production of 1,3-PD from various biomass resources. |
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