Measurement of pectin methylation in plant cell walls |
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| Authors: | RF McFeeters SA Armstrong |
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| Institution: | 1. Food Fermentation Laboratory, United States Department of Agriculture, Agricultural Research Service, North Carolina State University, Raleigh, North Carolina 27695 USA;2. North Carolina Agricultural Research Service, Department of Food Science, North Carolina State University, Raleigh, North Carolina 27695 USA |
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| Abstract: | A procedure was developed to measure the degree of pectin methylation in small samples of isolated cell walls from nonlignified plant tissues or pectin solutions. Galacturonic acid was determined colorimetrically with the 3,5-dimethylphenol reagent. Methylation was measured by base hydrolysis of galacturonic acid methyl esters, followed by gas chromatographic determination of released methanol. Estimates of the precision of analysis of pectin and cell wall samples were made. The coefficient of variation for estimates of the pectin esterification in cell walls isolated from 10-g samples of cucumber tissue ranged from 7.7 to 13.2%. |
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| Keywords: | pectin cell wall galacturonic acid methanol degree of methylation gas-liquid chromatography |
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