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Plant regeneration from leaf explants of Glycine clandestina Wendl
Authors:T Hymowitz  N L Chalmers  S H Costanza  M M Saam
Institution:(1) Department of Agronomy, University of Illinois, 61801 Urbana, IL, USA
Abstract:Fully fertile plants with the expected chromosome number 2n=40 were regenerated from excised leaf sections of Glycine clandestina. Shoots formed directly on the explants through organogenesis. Utilizing the same media and procedures fully fertile plants were also regenerated from cotyledon and hypocotyl tissue of the same G. clandestina accession. We were not successful in regenerating plants from root tissue of G. clandestina.Abbreviations 6-BA 6-Benzyladenine - FAA Formalin - NAA Naphthalenacetic acid - IAA Indoleacetic acid - Na2EDTA Disodium salt Ethylenediamine tetraacetic acid - Fe-NaEDTA Ferric-Sodium salt Ethylenediamine tetraacetic acid
Keywords:
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