A protoplast to plant system for the chrysanthemum Dendranthema zawadskii x D. grandiflora |
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Authors: | Graeme C Lindsay Susan E Ledger |
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Institution: | (1) MAF Technology, Ministry of Agriculture and Fisheries, Horticultural Research Centre, Private Bag, Levin, New Zealand;(2) Present address: Dept. of Cellular and Molecular Biology, nUniversity of Auckland, Private Bag, Auckland, New Zealand |
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Abstract: | Summary Plantlets were regenerated from protoplasts of in vitro shoot cultures and leaf-derived de novo shoots of the chrysanthemum Dendranthema zawadskii x D. grandiflora. Isolated protoplasts reformed cell walls and then began to divide within 24 hours of culture in streaky plate agarose lenses surrounded by liquid V-KM medium. Twenty one days after isolation, 1 mm diameter callus clumps were transferred to shoot regeneration medium. After a further 33 days leaves became visible. Elongated shoots were rooted on half strength hormone-free MS medium.Abbreviations BAP
6-benzylaminopurine
- IAA
3-indoleacetic acid
- MS
Murashige and Skoog (1962)
- NAA
1-naphthylacetic acid
- Pfr
Photon fluence rate
- V-KM
Binding and Nehls (1977) |
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Keywords: | Chrysanthemum Dendranthema zawadskii x D grandiflora Protoplast Regeneration |
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