Determination of surfactant critical micelle concentration by a novel fluorescence depolarization technique |
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| Affiliation: | 1. Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Universiteitsweg 99, 3508 TB Utrecht, Netherlands;2. A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Neulaniementie 2, Kuopio FIN-70211, Finland;3. Departamento de Química Orgánica, Universidad Autónoma de Madrid (UAM), Cantoblanco, 28049 Madrid, Spain;4. CosmoPHOS Ltd, 77 Tsimiski str., GR-54622 Thessaloniki, Greece;5. Heart Center and Gene Therapy Unit, Kuopio University Hospital, 70211 Kuopio, Finland;6. Institute for Advanced Research in Chemical Sciences (IAdChem), UAM, 28049 Madrid, Spain.;7. Instituto Madrileño de Estudios Avanzados (IMDEA)-Nanociencia c/Faraday, 9, Cantoblanco, 28049 Madrid, Spain |
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| Abstract: | ![]()
A novel method using fluorescence depolarization to determine the critical micelle concentrations (CMC) of surfactants was developed. Fluorescence anisotropies of Triton X-100, sodium dodecyl sulfate, and sodium cholate were measured using 1,6-diphenyl-1,3,5-hexatriene as a fluorescence probe. Fluorescence anisotropy decreased with increasing surfactant concentrations below the CMC and leveled off above the CMC. The depolarization method does not depend on the concentration of DPH and is largely immune to light-scattering problems encountered in turbid aqueous systems. |
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