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Contribution of mobile genetic elements to Desulfovibrio vulgaris genome plasticity |
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| Authors: | Christopher B. Walker Sergey Stolyar Dylan Chivian Nicolas Pinel Jeffrey A. Gabster Paramvir S. Dehal Zhili He Zamin Koo Yang Huei-Che B. Yen Jizhong Zhou Judy D. Wall Terry C. Hazen Adam P. Arkin David A. Stahl |
| Affiliation: | Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.; Virtual Institute for Microbial Stress and Survival ().; Physical Biosciences Division, Lawrence Berkeley National Laboratory , Berkeley, CA, USA.; Institute for Environmental Genomics, Oklahoma University, Norman, OK, USA.; Biochemistry Division, University of Missouri, Columbia, MO, USA.; Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. |
| Abstract: | The genome of Desulfovibrio vulgaris strain DePue, a sulfate-reducing Deltaproteobacterium isolated from heavy metal-impacted lake sediment, was completely sequenced and compared with the type strain D. vulgaris Hildenborough. The two genomes share a high degree of relatedness and synteny, but harbour distinct prophage and signatures of past phage encounters. In addition to a highly variable phage contribution, the genome of strain DePue contains a cluster of open-reading frames not found in strain Hildenborough coding for the production and export of a capsule exopolysaccharide, possibly of relevance to heavy metal resistance. Comparative whole-genome microarray analysis on four additional D. vulgaris strains established greater interstrain variation within regions associated with phage insertion and exopolysaccharide biosynthesis. |
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