Fermentation of 1-hexadecene by Candida lipolytica

Candida lipolytica, strain Phaff, was grown on 1.0% 1-hexadecene as sole source of carbon. Several oxidative intermediates were isolated and identified. Based on these intermediates two pathways are proposed for the degradation of the 1-alkene via the methyl group and the double bond. Subterminal oxidation of the 1-alkene was also indicated. Cell yield, lipid content, fatty acid profile and 1, 2-diol concentration are given for various rates of aeration during growth in a fermentor.