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Isolation and sequence of cDNA encoding the soybean protease inhibitors PI IV and C-II |
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| Authors: | P E Joudrier D E Foard L A Floener B A Larkins |
| Institution: | (1) Department of Botany and Plant Pathology, Purdue University, 47907 West Lafayette, IN, USA;(2) Present address: Institut National de la Recherche Agronomique, Laboratoire de Technologie des Céréales, 9 place Viala 34060 Montpellier Cedex, France;(3) Present address: Istituto Agronomico per l'Oltremare, Via Cocchi 4, 50131 Firenze, Italy;(4) Present address: Department of Entomology, Purdue University, 47907 West Lafayette, IN, USA |
| Abstract: | Two full-length (or nearly so) cDNA clones containing information for the protease inhibitors PI IV and C-II from soybean seeds were identified by means of a synthetic probe. DNA sequencing revealed that the two protease inhibitors are synthesized as precursors with a short peptide leader. The coding regions of the two clones show 80% homology, wheraes the 5 non-coding regions are 90% homologous. Homology of 75% is found in the region extending beyond the stop codons. |
| Keywords: | soybean protease inhibitor Bowman-Birk family cDNA sequence |
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