Isolation and Characterization of a Bacteriophage of Arthrobacter globiformis |
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| Authors: | K H Einck P A Pattee J G Holt C Hagedorn J A Miller and D L Berryhill |
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| Institution: | Department of Bacteriology, Iowa State University, Ames, Iowa 500100;Department of Bacteriology, North Dakota State University, Fargo, North Dakota 58102 |
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| Abstract: | A bacteriophage which reproduces on Arthrobacter globiformis ATCC 8010 was isolated from soil. This bacteriophage, designated phiAG8010, propagates either in soft agar or broth cultures of the host. Because of a slow adsorption rate, neither the latent period nor burst size was determined. The mature virion belongs to Bradley's group B and exhibits a hexagonal head measuring 69 nm (length) by 60 nm (width) attached to a sheathless tail 120 nm long. The buoyant density of the mature virion is 1.534 g/cm(3). The mature virion contains double-stranded DNA with a buoyant density of 1.722 g/cm(3) (equivalent to 63.3% G + C). Of 14 strains (representing 13 species) of Arthrobacter examined, including A. globiformis ATCC 4336, only A. globiformis ATCC 8010 supported replication of phiAG8010. |
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